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I came across an issue that seems to be common but I can't solve using previously suggested answers. My problem is as described in the title, I initially ran roary as follows: roary -e --mafft *.gff3 and got the error message. After some reading, a user suggested to run:
roary -e --mafft -z *.gff3 to keep temporary files but that doesn't change the result. In fact, the temporary file that is looked for in the temporary folder is DNA16.gff.proteome.faa but I have DNA16.gff.proteome.faa.tmp.filtered instead.
The file is a protein multifasta.
I've also tried running the command with sudo but nothing changed.
While checking the dependencies I get an error with the cd-hit version which should be higher than 4.6 even if I have 4.8.1, this makes me think this might be the cause of everything but I can't seem to be able to fix this: i) A fresh apt-get install didn't solve the issue, ii) conda install roary gets stuck at
Collecting package metadata
and eventually returns
Solving environment: failed with repodata from current_repodata.json, will retry with next repodata source.
In the past I came across a similar issue with Snippy in which a minor change to the source code made the dependencies version detection run smoothly, however I'm not sure if and how I should do it in this case.
Thanks a lot for any orientation and help.
The text was updated successfully, but these errors were encountered:
Dear all,
I came across an issue that seems to be common but I can't solve using previously suggested answers. My problem is as described in the title, I initially ran roary as follows:
roary -e --mafft *.gff3
and got the error message. After some reading, a user suggested to run:roary -e --mafft -z *.gff3
to keep temporary files but that doesn't change the result. In fact, the temporary file that is looked for in the temporary folder is DNA16.gff.proteome.faa but I have DNA16.gff.proteome.faa.tmp.filtered instead.The file is a protein multifasta.
I've also tried running the command with sudo but nothing changed.
While checking the dependencies I get an error with the cd-hit version which should be higher than 4.6 even if I have 4.8.1, this makes me think this might be the cause of everything but I can't seem to be able to fix this: i) A fresh
apt-get install
didn't solve the issue, ii)conda install roary
gets stuck atand eventually returns
In the past I came across a similar issue with Snippy in which a minor change to the source code made the dependencies version detection run smoothly, however I'm not sure if and how I should do it in this case.
Thanks a lot for any orientation and help.
The text was updated successfully, but these errors were encountered: